Title :
Quantifying focal disruptions in axonal microtubules
Author :
Dastgheyb, R.M. ; Barbee, K.A. ; Gallo, G.
Author_Institution :
Sch. of Biomed. Eng. & Health Sci., Drexel Univ., Philadelphia, PA, USA
Abstract :
Focal disruptions in axonal microtubules are a common consequence of Traumatic Brain Injuries (TBIs), and the ability to quantify these disruptions is essential to evaluating in vitro injury models and therapies. Here, we develop and implement an algorithm to demonstrate the ability to quantify focal disruptions in the microtubules of cultured neurons. The algorithm is implemented using a combination of ImageJ for pre-processing and Matlab for axon analysis and tubulin breakdown identification.
Keywords :
biomedical optical imaging; brain; cellular biophysics; fluorescence; image segmentation; injuries; mathematics computing; medical image processing; molecular biophysics; neurophysiology; proteins; ImageJ preprocessing; Matlab; Traumatic Brain Injuries; axon analysis; axonal microtubules; cultured neurons; focal disruptions; in vitro injury models; therapies; tubulin breakdown identification; Brain injuries; Calcium; Electric breakdown; MATLAB; Nerve fibers;
Conference_Titel :
Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast
Print_ISBN :
978-1-4799-8358-2
DOI :
10.1109/NEBEC.2015.7117155
