DocumentCode
3591296
Title
Quantifying focal disruptions in axonal microtubules
Author
Dastgheyb, R.M. ; Barbee, K.A. ; Gallo, G.
Author_Institution
Sch. of Biomed. Eng. & Health Sci., Drexel Univ., Philadelphia, PA, USA
fYear
2015
Firstpage
1
Lastpage
2
Abstract
Focal disruptions in axonal microtubules are a common consequence of Traumatic Brain Injuries (TBIs), and the ability to quantify these disruptions is essential to evaluating in vitro injury models and therapies. Here, we develop and implement an algorithm to demonstrate the ability to quantify focal disruptions in the microtubules of cultured neurons. The algorithm is implemented using a combination of ImageJ for pre-processing and Matlab for axon analysis and tubulin breakdown identification.
Keywords
biomedical optical imaging; brain; cellular biophysics; fluorescence; image segmentation; injuries; mathematics computing; medical image processing; molecular biophysics; neurophysiology; proteins; ImageJ preprocessing; Matlab; Traumatic Brain Injuries; axon analysis; axonal microtubules; cultured neurons; focal disruptions; in vitro injury models; therapies; tubulin breakdown identification; Brain injuries; Calcium; Electric breakdown; MATLAB; Nerve fibers;
fLanguage
English
Publisher
ieee
Conference_Titel
Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast
Print_ISBN
978-1-4799-8358-2
Type
conf
DOI
10.1109/NEBEC.2015.7117155
Filename
7117155
Link To Document