Fungistatic Activity and Identification of Antagonistic Actinomycetes to Camellia Disease from Soil

There are some effective microorganisms in the arboretum soil to inhibit the plant pathogenic fungus. This work mainly studied the actinomycetes from soil, which can inhibit Camellia oleifera pathogenic fungus. The antagonistic actinomyces strain F10 was isolated from rhizosphere soil of garden plants in Hengyang,Hunan province. The fungistatic activity of F10 was determined by confronting incubation on PDA plates and control test to the plants in house. The results showed that the strain F10 displayed a broad-spectrum inhibition activity to all the 8 plants pathogenic fungi tested. Especially, the inhibition rate of the strain F10 and its fermentation liquid to the Colletotrichum gloeosporioides, Fusarium proliferatum and Agaricodochium camellia was up to 80%; to Corticium sp., Exobasidium Vexans, C. gloeosporioides and Colletotrichum sp, the inhibition rate reached 50%. But the inhibition rate of F10´s fermentation filtrate was 36.2%, the fungistatic activity was not significant. According to the morphological and culture characteristics, and 16S rDNA ITS sequences analysis, the strain F10 was identified as Streptomyces globisporus. The results will be helpful to relieve the pressure which chemical pesticides brought a series of pollution problems to soil environment.