DocumentCode :
3595005
Title :
Ultrasonic enhancement of αvβ3 expressing-cells with targeted contrast agents
Author :
Dayton, Paul A. ; Pearson, David ; Clark, Jarrod ; Simon, Scott ; Schumann, Patricia ; Zutshi, Reena ; Matsunaga, Terry ; Ferrara, Katherine W.
Author_Institution :
Dept. of Biomed. Eng., Calfornia Univ., Davis, CA, USA
Volume :
1
fYear :
2003
Firstpage :
540
Abstract :
Targeted contrast agents have the potential to substantially improve the ability of ultrasound contrast agents to delineate otherwise difficult-to-detect pathologies. In this manuscript, optical microscopy and acoustic backscatter measurements from human umbilical vein endothelial cells (HUVEC) exposed to targeted and control microbubbles illustrate the high affinity of targeted agents for the αvβ3 integrin. Backscatter measurements are provided for cell layers exposed to three types of targeted contrast agents in addition to two controls. Acoustic studies illustrate a backscatter amplitude increase from αvβ3 expressing cells exposed to the targeted contrast agent of up to 13 fold compared to non-targeted agents. Additionally, echo decorrelation is shown to be a function of acoustic pressure and microbubble density. Frequency domain analysis demonstrates that adherent targeted microbubbles exhibit a significantly more narrowband spectrum than free microbubbles. These results suggest that targeted contrast agents may provide additional sensitivity in the detection of tumors, and that adherent contrast agents may be differentiable from free-floating microbubbles.
Keywords :
acoustic variables measurement; backscatter; biomedical ultrasonics; cellular biophysics; decorrelation; echo; frequency-domain analysis; medical signal detection; optical microscopy; tumours; αvβ3 expressing cells; acoustic backscatter measurements; acoustic pressure; cell layers; echo decorrelation; free floating microbubbles; frequency domain analysis; human umbilical vein endothelial cells; microbubble density; nontargeted agents; optical microscopy; targeted contrast agents; tumors detection; ultrasonic enhancement; ultrasound contrast agents; Acoustic measurements; Anthropometry; Backscatter; Humans; Optical microscopy; Optical sensors; Pathology; Ultrasonic imaging; Ultrasonic variables measurement; Veins;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Ultrasonics, 2003 IEEE Symposium on
Print_ISBN :
0-7803-7922-5
Type :
conf
DOI :
10.1109/ULTSYM.2003.1293461
Filename :
1293461
Link To Document :
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