Title :
Functionalised polyaniline nanowires: A prospective biosensing platform
Author :
Gangopadhyay, Rupali ; Chowdhury, Ankan Dutta ; De, Amitabha
Author_Institution :
Centre for Adv. Mater., Indian Assoc. for the Cultivation of Sci., Kolkata, India
Abstract :
In this work we have used functionalized polyaniline (PAn) nanowires for fabrication of a biosensing platform which was exploited for fabrication of three different biosensors. In one effort, we have immobilized single-stranded DNA oligonucletide (ssdA) on this platform and have monitored its combination with the complementary strand (ssdT) at a concentration as low as 10-18 M. Moreover we have immobilized glucose oxidase on this platform that could be used for amperometric detection of glucose in aqueous solution over a wide concentration range of 1μM-20mM. We could successfully immobilize Lamin A (LA) antibody on the platform and its response towards the Lamin A protein could be nicely demonstrated down to a concentration as low as 10 μm. Therefore, the efficiency of the general biosensing platform is established.
Keywords :
DNA; amperometric sensors; biosensors; conducting polymers; enzymes; molecular biophysics; nanobiotechnology; nanofabrication; nanosensors; nanowires; Lamin A antibody; Lamin A protein; amperometric detection; aqueous solution; complementary strand DNA oligonucletide; functionalised polyaniline nanowires; immobilized glucose oxidase; immobilized single-stranded DNA oligonucletide; prospective biosensing platform; Biosensors; DNA; Electrodes; Molecular biophysics; Nanowires; Proteins; Sugar; Lamin A; biosensor; glucose oxidase; polyaniline; ss-DNA;
Conference_Titel :
Emerging Electronics (ICEE), 2014 IEEE 2nd International Conference on
Print_ISBN :
978-1-4673-6527-7
DOI :
10.1109/ICEmElec.2014.7151161
