Title :
Development of MAb-based Immunochromatographic Assay for Cadmium from Biological Samples
Author :
Sasaki, Kazuhiro ; Tawarada, Kei ; Okuhata, Hirosi ; Miyasaka, Hitoshi ; Okuyama, Akira ; Maruyama, Yukinao ; Ohmura, Naoya
Author_Institution :
Central Res. Inst. of Electr. Power Ind.
Abstract :
Cadmium (Cd) is a general environmental pollutant of increasing global concern. In 2005 the joint FAO/WHO Codex Alimentarius Commission proposed new international food legislation for low-level Cd contaminants. In this study we demonstrate the use of novel monoclonal antibody (MAb) to Cd-EDTA in an immunochromatography (IC) format for the quick testing for trace Cd. This IC device could detect 0.3 mug kg-1 (0-3 ppb) Cd. Contaminated Zn, Mn, Mg, and Cu, which would interfere the measurement of Cd by cross reaction to the MAb, could be removed by using a column that could separate trace Cd from other heavy metals in the extract of brown rice
Keywords :
agricultural products; biochemistry; biosensors; cadmium; chromatography; molecular biophysics; pollution measurement; proteins; Cd; EDTA; FAO-WHO Codex Alimentarius Commission; MAb-based immunochromatographic assay; biological samples; brown rice; cadmium; cross reaction; environmental pollutant; international food legislation; monoclonal antibody; Cadmium; Cities and towns; Electronic equipment testing; Immune system; Integrated circuit testing; Laboratories; Lead; Mice; Powders; Proteins;
Conference_Titel :
Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE
Print_ISBN :
1-4244-0032-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2006.260444
