Title :
Peculiarities of DNA detection using evanescent field biosensing
Author :
Bgrchers, T. ; Spener, F. ; Specht, B. ; Kruchinin, A.A. ; Vlasov, Yu.G.
Author_Institution :
Inst. of Chem. & Biochem. Sensor Res., Munich, Germany
Abstract :
It is shown that the main peculiarities of DNA biosensing are related with electrical charge of nucleic acid molecule. The electrostatic interaction plays an important role in specificity of the sensor and can be the reason of strong steric hindrance for high length DNA fragments in negatively charged three dimensional matrix known as powerful tool for detection of proteins. Therefore, the design of recognition element for rather long products of polymerase chain reaction (PCR) needs its own strategy different from one optimized for proteins
Keywords :
DNA; biosensors; electric charge; molecular biophysics; DNA detection; biosensor; detection of proteins; electrical charge; electrostatic interaction; evanescent field biosensing; high length DNA fragments; negatively charged three dimensional matrix; nucleic acid molecule; polymerase chain reaction; recognition element; steric hindrance; Biosensors; Chemical and biological sensors; Chemical sensors; Chemistry; DNA; Design optimization; Electrostatics; Polymers; Proteins; Sensor phenomena and characterization;
Conference_Titel :
Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on
Print_ISBN :
0-7803-3829-4
DOI :
10.1109/SENSOR.1997.613694
