Diffuse double layer effects observed by dielectrophoresis of Herpes Simplex Virus, type 1 (HSV-1)

Author

Hughes, Michael P. ; Morgan, Hywel

Author_Institution

Biomed. Eng. Group, Surrey Univ., Guildford, UK

Volume

1

fYear

1999

fDate

6/21/1905 12:00:00 AM

Abstract

Dielectrophoresis is often used for characterisation of bioparticles on the cellular and subcellular scale. Dielectrophoretic crossover analysis enables the determination of biophysical properties of such particles. The DEP properties of colloidal particles (less than one micrometre diameter) are strongly dependent on the surface particle conductivity. Recent work has demonstrated how the effective conductivity of small latex spheres contains components due to both Stern layer and diffuse double layer. Here we demonstrate that this model can also be used to account for the DEP behaviour of virus particles such as Herpes Simplex (HSV-1)

Keywords

bioelectric phenomena; cellular biophysics; colloids; electrophoresis; microorganisms; HSV-1; Herpes Simplex Virus; colloidal particles; dielectrophoresis; dielectrophoretic crossover analysis; diffuse double layer effects; relative permittivity; Biomembranes; Conductivity; Dielectrophoresis; Frequency; Microorganisms; Permittivity; Polarization; Proteins; Surface fitting; Viruses (medical);

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802125

Filename

802125