Title :
Fast Axial-Scanning Widefield Microscopy With Constant Magnification and Resolution
Author :
Martinez-Corral, Manuel ; Po-Yuan Hsieh ; Doblas, Ana ; Sanchez-Ortiga, Emilio ; Saavedra, Genaro ; Yi-Pai Huang
Author_Institution :
Dept. of Opt., Univ. of Valencia, Burjassot, Spain
Abstract :
In this paper, we propose the use of electrically-addressable lens devices for performing fast non-mechanical axial scanning when imaging three-dimensional samples. This non-mechanical method can be implemented in any commercial microscope. The approach is based on the insertion of the tunable lens at the aperture stop of the microscope objective. By tuning the voltage, a stack of depth images of 3D specimens can be captured in real time and with constant magnification and resolution. The main advantage of our technique is the possibility of performing fast axial scanning free of mechanical vibrations.
Keywords :
lenses; optical microscopy; optical tuning; vibrations; constant magnification; electrically-addressable lens devices; fast axial-scanning wide field microscopy; mechanical vibrations; nonmechanical axial scanning; resolution; three-dimensional samples; tuning; Apertures; Lenses; Liquids; Microscopy; Optical imaging; Optical microscopy; Three-dimensional displays; Axial scanning; liquid crystal lens; liquid lens; telecentricity; three-dimensional (3D) microscopy;
Journal_Title :
Display Technology, Journal of
DOI :
10.1109/JDT.2015.2404347