Multilevel Regulation and Translational Switches in Synthetic Biology

Author

Kopniczky, Margarita B. ; Moore, Simon J. ; Freemont, Paul S.

Author_Institution

Dept. of Med., Imperial Coll. London, London, UK

Volume

9

Issue

4

fYear

2015

Firstpage

485

Lastpage

496

Abstract

In contrast to the versatility of regulatory mechanisms in natural systems, synthetic genetic circuits have been so far predominantly composed of transcriptionally regulated modules. This is about to change as the repertoire of foundational tools for post-transcriptional regulation is quickly expanding. We provide an overview of the different types of translational regulators: protein, small molecule and ribonucleic acid (RNA) responsive and we describe the new emerging circuit designs utilizing these tools. There are several advantages of achieving multilevel regulation via translational switches and it is likely that such designs will have the greatest and earliest impact in mammalian synthetic biology for regenerative medicine and gene therapy applications.

Keywords

RNA; cellular biophysics; gene therapy; molecular biophysics; proteins; gene therapy; mammalian synthetic biology; multilevel regulation; post transcriptional regulation; protein; regenerative medicine; ribonucleic acid; synthetic genetic circuits; translational regulators; translational switches; Circuit synthesis; Gene expression; Logic gates; Protein engineering; Proteins; RNA; Synthetic biology; Biomedical engineering; circuit design; logic devices; protein; ribonucleic acid (RNA); riboswitch; synthetic biology; translation;

fLanguage

English

Journal_Title

Biomedical Circuits and Systems, IEEE Transactions on

Publisher

ieee

ISSN

1932-4545

Type

jour

DOI

10.1109/TBCAS.2015.2451707

Filename

7226874