Miniaturized Transmission-Line Sensor for Broadband Dielectric Characterization of Biological Liquids and Cell Suspensions

The nondestructive characterization of biological liquids and cell suspensions using electromagnetic waves in the microwave frequency range calls for accurate and sensitive measurement devices. Especially when reducing the sample volume, the sensitivity becomes a critical design parameter for broadband sensors. In this paper, a miniaturized transmission-line sensor based on a coplanar waveguide is used to characterize the permittivity of nanoliter volumes of biologically relevant liquids and cell suspensions. The sensor´s sensitivity is increased by means of electrically small discontinuities within the sensing section. The biological samples are guided across the sensor in a microfluidic channel, which is fabricated using microsystems technology. The sensor is used between 850 MHz and 40 GHz to detect the broadband permittivity of liquid samples such as aqueous salt and protein solutions. The experimentally detected contrast between living and dead Chinese hamster ovary cells in suspension is significant despite the small sample volume.