Demonstration of fiber direction glycogen in the rat EDL muscle with longitudinal sections: Histological assessment

In this study, the distribution of glycogen in fiber direction is examined histologically in rat EDL muscle in order to investigate the intramuscular mechanism of botox. The muscle was not exposed to any physical activity or electrical stimulation; it was directly subjected to histological study. The longitudinal sections obtained from the paraffin-embedded tissue was stained by Periodic Acid and Schiff (PAS) solutions, afterwards hematoxylin Mayer´s and bluing agent was added. Under the influence of PAS, the areas containing glycogen was observed as blue and the nuclei was dark blue. Furthermore, fiber directions were also determined with the contribution of the second step. As a result of these studies it was observed that glycogen was distributed among the whole muscle in the fiber direction. Utilizing the obtained findings, botox injected muscle will be stained with PAS and paralyzed muscle fiber patterns will be determined. The mechanical effects of botox will be examined intramuscularly and the first results will be presented in the congress.