Interpulsed multifrequency electrical impedance measurements during electroporation of adherent differentiated myotubes

Plasma membrane of living cells constitutes the barrier between the intracellular and extracellular media and regulates the transport of chemical species from or into the cell cytoplasm. Electroporation, also called electropermeabilization, is a phenomenon occurring when cell membranes are exposed to high electric field pulses. When the parameters of such electric field are appropriate, a transient state of permeability to molecular species is generated. The technique is currently used as a tool to deliver membrane impermeable molecules into the cells both in vitro and in vivo with its main interest focused in electrochemotherapy of tumors and nucleic acids electrotransfer for gene therapy or DNA vaccination.