Investigation of the performance of variable-density Z-spectrum acquisition scheme in MR chemical exchange saturation transfer effect quantification

Chemical exchange saturation transfer (CEST) MRI emerges as a powerful imaging method to probe chemical exchange between bulk water and labile protons. Conventional CEST imaging needs detailed Z-spectrum acquisition, even at frequencies with limited information for CEST parameter quantification, which harms imaging efficiency and is not favorable in practical applications. In this study, a variable-density Z-spectrum acquisition scheme was proposed. Data with saturation frequencies close to water, labile protons and their negative frequency offset was densely sampled, while the remaining part was sparsely acquired. The accuracy of the proposed method in CEST ratio measurement was investigated with numerical simulation. Compared to the conventionally evenly distributed Z-spectrum acquisition method, the proposed scheme showed significant improvement of CEST ratio characterization with existence of noise and static magnetic field inhomogeneity. With the additional advantage of easy implementation, the developed Z-spectrum acquisition scheme provides a useful framework for accurate CEST parameter quantification without compromising the imaging time.