Femtosecond stimulated Raman Spectroscopy as a first step for nonlinear microscopy

In this paper, with the aim to realize a nonlinear microscope based on Coherent Raman Spectroscopy, we describe the implementation of femtosecond stimulated Raman scattering technique as a first step, i.e. single pixel, towards nonlinear microscope. Details of experimental set up and the main issue for experimental implementation of femtoseconds CRS spectroscopy are discussed. Finally, Stimulated Raman Scattering measurements on a bulky-polystyrene sample are reported.