Raman microscopy: Chemical and analytical imaging of biomolecules

Raman spectroscopy has been widely utilized to analyze various type of material. However the low scattering cross-section of Raman scattering prevents the application in microscopic imaging. In our research, we have developed a Raman microscope system with improved imaging speed, where using line-shaped illumination is used to detect Raman spectra simultaneously at multiple points in a sample. This improvement allowed us to utilize Raman imaging in biological research such as label-free hemeprotein imaging, diagnosis of cell differentiations.