Segmentation of Multicolor Fluorescence In-Situ Hybridization (M-FISH) image using an improved Fuzzy C-means clustering algorithm while incorporating both spatial and spectral information

Multicolor Fluorescence In-Situ Hybridization (M-FISH) is an imaging technique for rapid detection of chromosomal abnormalities, where the segmentation of chromosomes has been a challenge. Multi-channel information of M-FISH images can be used in a segmentation algorithm to exploit the correlated information across channels for better image segmentation. In addition, the neighboring pixels share similar characteristics, so this spatial information can be further utilized to improve the robustness of the algorithm to the noise. Motivated by this fact, in this paper we proposed an improved Fuzzy C-means (FCM) clustering algorithm to overcome the problems of conventional FCM such as the sensitivity to noise by incorporating both spatial and spectral information. The experimental results on both simulated and real M-FISH images have shown that our proposed method can result in higher segmentation accuracy and lower false ratio than both conventional FCM and the improved adaptive FCM (IAFCM) we recently proposed.