DNA barcodes for cultivar identification of Thailand Tamarindus indica.L

This study aims to investigated Tamarindus indica.L. chloroplast rbcL gene for ribulose-1,5-bisphosphate carboxylase/oxygenase, large subunit as the molecular marker to identified the cultivar level of Tamarindus indica.L. in Thailand. The partial rbcL gene of 19cultivars were sequences approximately 611-712 bp. The sequence alignment and Phylogenetic Tree analysis were generated by Clustal W and NJ methods. The result of nucleotide sequence alignment of partial rbcL gene for all cultivars were divided into 19 groups. The different of these groups similar to the different of local name of Tamarind cultivars in Phetchabun Province, Thailand. Base on these results indicated that 19 cultivar DNA Barcodes can supported the local opinions act as the scientific data to identification of tamarind cultivars in Thailand.