A two-dimensional fluorometric imaging "sniffer camera" of ethanol vapor for evaluation of alcohol metabolism using enzymatic reaction

Various volatile organic compounds can be found in human transpiration, breath and body odor. Therefore, a novel two-dimensional fluorometric imaging system, known as a "sniffer camera" for gaseous ethanol emissions from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multi UV-LED excitation sheet and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of NADH, which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed a rapidly and accurately responses and a visible measurement, which could lead an analysis to metabolism function at real time.