Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope

There has been much research about STED microscopy to reduce cost. In that microscopy, it is necessary to synchronize the pulsed lights which is just for initial setup. However, there has not been researched in order to reduce this expense until now. So, in this paper, we propose the low-cost strategy to measure the time delay in STED microscopy. To achieve that, we utilized TCSPC principle with a general digital oscilloscope. As a result, we attained successful measurement and adjustment about two pulsed lights with 180-ps delay in STED system.