Refined calibration methods for optical potential mapping in single cardiomyocytes

Author

Platzer, D. ; Dapra, D. ; Windisch, H.

Author_Institution

Inst. fur Medizinische Phys. und Biophys., Karl-Franzens-Univ., Graz, Austria

Volume

1

fYear

2001

fDate

2001

Firstpage

283

Abstract

Fluorescence imaging provides a comprehensive view of the electrical behavior of excitable cells, the relative nature of the optical signals however requires additional means for calibration. The determination of the actual electrical field strength is subject to uncertainty even when using concurrent electrode measurements. A combined approach of electrical field measurement and optical mapping of the spatially distributed polarizations provides a satisfying calibration of the optical signals in our field-stimulation experiments.

Keywords

bio-optics; bioelectric potentials; biological techniques; calibration; cardiology; cellular biophysics; fluorescence; muscle; action potential; cardiac activation; concurrent electrode measurements; electric field-stimulation; excitable cells electrical behavior; field-stimulation experiments; fluorescent dye; optical potential-mapping; spatially distributed polarizations; Biomedical optical imaging; Biomembranes; Calibration; Cardiology; Electric variables measurement; Fluorescence; Optical filters; Optical recording; Optical sensors; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE

ISSN

1094-687X

Print_ISBN

0-7803-7211-5

Type

conf

DOI

10.1109/IEMBS.2001.1018913

Filename

1018913