Title :
Refined calibration methods for optical potential mapping in single cardiomyocytes
Author :
Platzer, D. ; Dapra, D. ; Windisch, H.
Author_Institution :
Inst. fur Medizinische Phys. und Biophys., Karl-Franzens-Univ., Graz, Austria
Abstract :
Fluorescence imaging provides a comprehensive view of the electrical behavior of excitable cells, the relative nature of the optical signals however requires additional means for calibration. The determination of the actual electrical field strength is subject to uncertainty even when using concurrent electrode measurements. A combined approach of electrical field measurement and optical mapping of the spatially distributed polarizations provides a satisfying calibration of the optical signals in our field-stimulation experiments.
Keywords :
bio-optics; bioelectric potentials; biological techniques; calibration; cardiology; cellular biophysics; fluorescence; muscle; action potential; cardiac activation; concurrent electrode measurements; electric field-stimulation; excitable cells electrical behavior; field-stimulation experiments; fluorescent dye; optical potential-mapping; spatially distributed polarizations; Biomedical optical imaging; Biomembranes; Calibration; Cardiology; Electric variables measurement; Fluorescence; Optical filters; Optical recording; Optical sensors; Voltage;
Conference_Titel :
Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE
Print_ISBN :
0-7803-7211-5
DOI :
10.1109/IEMBS.2001.1018913
