Nano-ampere stimulation window for cultured neurons on micro-electrode arrays

Author

Buitenweg, J.R. ; Rutten, W.L.C. ; Marani, E.

Author_Institution

Inst. for Biomed. Technol., Twente Univ., Enschede, Netherlands

Volume

1

fYear

2001

fDate

2001

Firstpage

725

Abstract

From experiments, it appears to be possible to stimulate a neuron by depolarisation of the lower membrane patch, the sealing part of the membrane, using a nano-ampere current through the extracellular electrode. Also, a stimulation window is observed. These findings can be explained by a finite element model of the neuron-electrode interface which permits geometry based dynamic modeling of the neuron-electrode interface and can be used to explore the requirements for lower membrane stimulation in more detail. An intracellular action potential develops if the current through the lower membrane is able to depolarize the upper membrane sufficiently.

Keywords

arrays; bioelectric potentials; biological techniques; biomembranes; cellular biophysics; microelectrodes; neurophysiology; cultured neurons; current densities; extracellular stimulation; interface geometry; local membrane potentials; long term bioelectrical activity recording; multielectrode arrays; neuron-electrode contact; neuroscience method; sealing; single cell electrical contacts; Bioelectric phenomena; Biomembranes; Conductors; Electric potential; Electrodes; Extracellular; Finite element methods; Geometry; Neurons; Solid modeling;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE

ISSN

1094-687X

Print_ISBN

0-7803-7211-5

Type

conf

DOI

10.1109/IEMBS.2001.1019043

Filename

1019043