Title :
Survival of adhering cortical neurons on polyethylenimine micropatterns
Author :
Ruardij, T.G. ; Goedbloed, M.H. ; Rutten, W.L.C.
Author_Institution :
Fac. of Electr. Eng., Twente Univ., Enschede, Netherlands
Abstract :
The influence of neuron-adhesive pattern geometry on long-term survival of cortical neural tissue (rat brain) was studied over a time period of 15 days. Microwells (depth 0.5 μm) with diameters of 25, 50, 100 and 150 μm and inter-microwell distances of 15, 30, 60 and 90 μm, were etched in a neuron-repellent fluorocarbon (FC)-layer and coated with neuron-adhesive polyethylenimine (PEI). Results showed that the survival of neural tissue was geometry-independent after 1, 4 and 8 days but was favored on 150 μm wells after 15 days.
Keywords :
adhesion; biological techniques; biomedical electrodes; brain; cellular biophysics; microelectrodes; neurophysiology; axonal sprouts; cortical neural tissue; cultured neuron probe; long-term survival; microelectrodes; microwells; miniaturization; neuron-adhesive pattern geometry; neuron-adhesive polyethylenimine; neuron-repellent fluorocarbon; photolithography; rat brain; viability; Adhesives; Chemicals; Electrodes; Etching; Geometry; Neurons; Plasma applications; Plasma chemistry; Probes; Resists;
Conference_Titel :
Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE
Print_ISBN :
0-7803-7211-5
DOI :
10.1109/IEMBS.2001.1019049
