Title :
Kinetic studies of molecular recognition on DNA by using a 27 MHz QCM
Author_Institution :
Dept. of Biomolecular Eng., Tokyo Inst. of Technol., Yokohama, Japan
Abstract :
We introduce a 27-MHz quartz-crystal microbalance (QCM) as a new tool to detect DNA-protein/peptide interactions in an aqueous solution. We undertook observations of a various protein/peptide (for example, histone and transcription factors such as a zinc-finger type peptide or a leucine-zipper type peptide) binding onto double strand DNA (dsDNA) included their binding site.
Keywords :
DNA; biological techniques; crystal oscillators; microbalances; molecular biophysics; proteins; 27 MHz; DNA-protein/peptide interactions; Zn finger type peptide; aqueous solution; binding site; double strand DNA; histone; kinetic studies; leucine-zipper type peptide; molecular recognition; quartz-crystal microbalance; transcription factors; Biochemistry; Biological control systems; Biological processes; DNA; Frequency; Gene expression; Kinetic theory; Peptides; Protein engineering; Sequences;
Conference_Titel :
Sensors, 2002. Proceedings of IEEE
Print_ISBN :
0-7803-7454-1
DOI :
10.1109/ICSENS.2002.1037092
