A lab-on-a-chip for cell detection and manipulation

Author

Medoro, G. ; Manaresi, N. ; Leonardi, A. ; Altomare, L. ; Tartagni, M. ; Guerrieri, R.

Author_Institution

Silicon Biosystems, Bologna, Italy

Volume

1

fYear

2002

fDate

2002

Firstpage

472

Abstract

This paper presents a lab-on-a-chip for electronic manipulation and detection of microorganisms based on the use of closed dielectrophoretic (DEP) cages combined with impedance sensing. A prototype has been realized using a standard printed circuit board (PCB) technology by which polystyrene microbeads have been trapped, concentrated and quantitated, in agreement with CAD simulations, without the need for external optical components. The experiment was successfully repeated with S. cerevisiae. The approach is suited to be implemented in integrated circuit technology, which would allow to manipulate and detect single cells and reduce the system dimension.

Keywords

bioelectric phenomena; cellular biophysics; electrophoresis; laboratory techniques; microorganisms; CAD simulations; S. cerevisiae; biophysical research instrumentation; dielectrophoresis; electronic manipulation; external optical components; impedance sensing; microorganisms detection; polystyrene microbeads; single cells; standard printed circuit board technology; system dimension reduction; Charge carrier processes; Circuit simulation; Dielectrophoresis; Electron traps; Impedance; Integrated circuit technology; Lab-on-a-chip; Microorganisms; Printed circuits; Virtual prototyping;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2002. Proceedings of IEEE

Print_ISBN

0-7803-7454-1

Type

conf

DOI

10.1109/ICSENS.2002.1037139

Filename

1037139