In-vivo applications of microscale digital particle image velocimetry

Author

Acevedo-Bolton, G. ; Hove, J.R. ; Koeste, R. ; Forouhar, A. ; Frase, S.E. ; Gharib, M.

Author_Institution

Option of Bioeng., California Inst. of Technol., Pasadena, CA, USA

Volume

2

fYear

2002

fDate

2002

Firstpage

1303

Abstract

Digital particle image velocimetry (DPIV) is an extremely powerful technique for quantitative flow measurements. In order to perform hemodynamic measurements in embryonic hearts, DPIV analysis was performed on both Zebrafish (Danio rerio) and Quail (Coturnix coturnix japonica) embryos. A protocol for performing DPIV at the microscale with microscopy and high-speed imaging is presented. Results show the presence of vortices and jets at low Reynolds numbers implying a high shear on vessel walls, and suggest that hemodynamics may play an important role in future heart development studies.

Keywords

biological techniques; blood flow measurement; cardiology; genetics; jets; vortices; Coturnix coturnix japonica; Danio rerio; Quail embryos; Zebrafish embryos; cardiogenesis; embryonic hearts; genetic programming; heart development; hemodynamic measurements; high shear; high-speed imaging; in-vivo applications; jets; low Reynolds numbers; microscale; microscale digital particle image velocimetry; microscopy; protocol; quantitative flow measurements; vessel walls; vortices; Blood; Cardiology; Cells (biology); Embryo; Heart; Hemodynamics; Microscopy; Performance analysis; Performance evaluation; Stress;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1106399

Filename

1106399