Title :
In-vivo applications of microscale digital particle image velocimetry
Author :
Acevedo-Bolton, G. ; Hove, J.R. ; Koeste, R. ; Forouhar, A. ; Frase, S.E. ; Gharib, M.
Author_Institution :
Option of Bioeng., California Inst. of Technol., Pasadena, CA, USA
Abstract :
Digital particle image velocimetry (DPIV) is an extremely powerful technique for quantitative flow measurements. In order to perform hemodynamic measurements in embryonic hearts, DPIV analysis was performed on both Zebrafish (Danio rerio) and Quail (Coturnix coturnix japonica) embryos. A protocol for performing DPIV at the microscale with microscopy and high-speed imaging is presented. Results show the presence of vortices and jets at low Reynolds numbers implying a high shear on vessel walls, and suggest that hemodynamics may play an important role in future heart development studies.
Keywords :
biological techniques; blood flow measurement; cardiology; genetics; jets; vortices; Coturnix coturnix japonica; Danio rerio; Quail embryos; Zebrafish embryos; cardiogenesis; embryonic hearts; genetic programming; heart development; hemodynamic measurements; high shear; high-speed imaging; in-vivo applications; jets; low Reynolds numbers; microscale; microscale digital particle image velocimetry; microscopy; protocol; quantitative flow measurements; vessel walls; vortices; Blood; Cardiology; Cells (biology); Embryo; Heart; Hemodynamics; Microscopy; Performance analysis; Performance evaluation; Stress;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1106399
