Flow induced platelet activation in mechanical heart valves - in vitro studies

Author

Yin, Wei ; Bluestein, Danny ; Jesty, Jolyon ; Perrotta, Peter

Author_Institution

Biomed. Eng. Dept., State Univ. of New York, Stony Brook, NY, USA

Volume

2

fYear

2002

fDate

2002

Firstpage

1599

Abstract

Thromboembolism is a common complication of mechanical heart valves (MHV). The objective of this study was to elucidate mechanisms of thromboembolism in flow past MHV in vitro. Gel-filtered platelets were recirculated through the MHV mounted in a model of left ventricular assist device (LVAD) and their activation was measured by using an innovative platelet activity state (PAS) assay. The motion of one MHV in LVAD was restricted by suture, and LVAD with free MHV was considered as control. The platelet activation increased as a function of the recirculation time past the valve, and the restricted valve had a higher activation rate as compared to controls. Flow cytometry was conducted to compare the results obtained from PAS assay.

Keywords

artificial organs; cardiology; haemodynamics; physiological models; prosthetics; valves; activation rate; flow cytometry; flow induced platelet activation; free MHV; gel-filtered platelets; in vitro studies; left ventricular assist device; mechanical heart valves; platelet activity state assay; recirculation time; restricted valve; suture; thromboembolism; Biomedical engineering; Biomedical measurements; Filtration; Heart valves; Hemorrhaging; In vitro; Motion control; Pathology; Plasma measurements; Prosthetics;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1106557

Filename

1106557