DocumentCode :
386404
Title :
Growth of cartilage explants in long-term: free-swelling culture: biochemical and tensile biomechanical properties
Author :
Williamson, Amanda K. ; Sah, Robert L.
Author_Institution :
Dept. of Bioeng., California Univ., San Diego, La Jolla, CA, USA
Volume :
1
fYear :
2002
fDate :
2002
Firstpage :
436
Abstract :
Articular cartilage mechanical properties and biochemical composition evolve during in utero development and in vivo growth, with marked differences between the fetus, newborn, and young adult. The objective of this study was to establish an in vitro system to assess the factors that regulate such growth, in particular by characterizing the growth of fetal and newborn bovine articular cartilage explants during 6 weeks of free-swelling culture in serum-supplemented medium. During this culture period, both fetal and calf cartilage grew markedly in size, increasing in cell and matrix components. While the tensile modulus, strength and stiffness of calf cartilage were initially higher than those of fetal cartilage, the tensile properties of both types of cartilage explants fell, to similarly low levels, during the culture period. The increase in cells and matrix, but diminution rather than increase in tensile properties, suggests that regulatory factors other than serum, may trigger the biomechanical maturation of articular cartilage in vivo.
Keywords :
biochemistry; biological tissues; biomechanics; cellular biophysics; elastic moduli; swelling; 6 w; biochemical properties; biomechanical maturation triggering; calf cartilage; cartilage explants growth; fetal cartilage; free-swelling culture; regulatory factors; tensile biomechanical properties; Analysis of variance; Biomedical engineering; Bovine; In vivo; Mechanical factors; Pediatrics; Residual stresses; Tensile strain; Tensile stress; Testing;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1136883
Filename :
1136883
Link To Document :
بازگشت