Fluid shear-induced membrane fluctuations measured by laser dark field microscopy

Author

Haidekker, M.A. ; Stevens, H.Y. ; Frangos, J.A.

Author_Institution

Dept. of Bioeng., California Univ., San Diego, La Jolla, CA, USA

Volume

1

fYear

2002

fDate

2002

Firstpage

584

Abstract

The mechanism of mechanochemical signal transduction remains to be elucidated. There is mounting evidence that mechanochemical transduction in response to fluid shear stress occurs at the cell membrane. We previously demonstrated that fluid shear stress increases membrane free volume. Here we present evidence that fluid shear amplifies thermal fluctuations in the cell membrane. These results present further support of the membrane free volume theory of mechanochemical transduction.

Keywords

biological techniques; biomembranes; biothermics; cellular biophysics; haemorheology; measurement by laser beam; optical microscopy; cell membrane; fluid shear-induced membrane fluctuations; laser dark field microscopy; mechanochemical transduction; mechanotransduction; membrane free volume increase; membrane thermal fluctuations; shear stress; Biomedical engineering; Biomembranes; Cells (biology); Fluctuations; Light scattering; Measurement by laser beam; Microscopy; Proteins; Surface morphology; Thermal stresses;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1136962

Filename

1136962