Title :
Design of a single capillary-parenchymal co-culture bioreactor
Author :
Kim, E.S. ; Kaazempur-Mofrad, M.R. ; Borenstein, J.T. ; Vacanti, J.P. ; Kamm, R.D.
Author_Institution :
Dept. of Mech. Eng., MIT, Cambridge, MA, USA
Abstract :
A bioreactor is designed and fabricated for the co-culture of endothelial and hepatic parenchymal cells. The device provides a structured environment mimicking the in vivo characteristics of a single capillary and the adjacent tissue. The bioreactor consists of two cell-scale fluid channels separated by a bio-compatible thin porous membrane made of self-assembling oligopeptide gel. Analytic and computational modeling techniques were used to investigate the fluid flow and mass transfer characteristics and aid in design of the bioreactor. Study of co-cultured cells in this device will contribute to the development of tissue-engineered organs.
Keywords :
biological techniques; blood vessels; cellular biophysics; gels; haemorheology; liver; physiological models; MEMS; bio-compatible thin porous membrane; computational modeling; endothelial cells; hepatic parenchymal cells; mass transfer characteristics; self-assembling oligopeptide gel; self-assembling peptide; single capillary-parenchymal co-culture bioreactor design; tissue-engineered organs development; Biomembranes; Bioreactors; Etching; Finite element methods; Glass; Pattern analysis; Peptides; Pumps; Tides; Viscosity;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1137106
