Fluid flow increases mineralized matrix deposition in three-dimensional perfusion culture of marrow stromal osteoblasts in a dose-dependent manner

Author

Sikavitsas, V.I. ; Bancroft, G.N. ; van den Dolde, J. ; Sheffield, T.L. ; Jansen, J.A. ; Ambrose, C.G. ; Mikos, A.G.

Author_Institution

Dept. of Bioeng., Rice Univ., Houston, TX, USA

Volume

1

fYear

2002

fDate

2002

Firstpage

884

Abstract

The aim of the study is to investigate the effect of flow perfusion in three-dimensional titanium meshes seeded with rat marrow stromal cells (MSCs), on the ability of these cells to proliferate, differentiate towards the osteoblastic lineage, and generate mineralized extracellular matrix. Flow perfusion allows the continuous infusion of cell culture media throughout the porous network of the scaffold. It also permits the mechanical stimulation of the seeded cells by exposing them to shear forces.

Keywords

biological techniques; biorheology; bone; calcium; cellular transport; 0.8 mm; 10 mm; 16 day; 4 day; 40 m; 40 mg; 8 day; Ca; Ti; cell culture media; extracellular matrix; fluid flow; marrow stromal osteoblasts; mechanical stimulation; mechanotransduction; mineralized matrix deposition; osteoblastic lineage; porous network; rat; shear forces; three-dimensional perfusion culture; three-dimensional titanium meshes; Biomedical engineering; Bones; Calcium; Cells (biology); Dentistry; Educational institutions; Extracellular; Fluid flow; Mineralization; Titanium;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1137126

Filename

1137126