Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells

Author

Lucarelli, E. ; Beccheroni, A. ; Vento, AM Del ; Reggiani, M. ; Sangiorgi, L. ; Bonaccorsi, L. ; Picci, P. ; Cenacchi, A. ; Meotti, C. ; Fornasari, P.M. ; Mercuri, M. ; Donat, D.

Author_Institution

Lab. di Ricerca Oncologica, Ist.Ortopedici Rizzoli, Bologna, Italy

fYear

2002

fDate

2002

Firstpage

155

Lastpage

156

Abstract

Mesenchymal stem cells (MSC) contained in the bone marrow provide an invaluable source for for bone reconstruction. Major limitations in their use are: the relative low number of MSC in the marrow cell population and the small volume of bone marrow available. There are emerging data demonstrating clinical applications of ex-vivo expanded cells, whose expansion is supported by several growth factors. We investigated whether growth factors (GFs) released by platelets stimulated MSC proliferation. MTT assays showed that the effect of GFs on MSC proliferation was dose dependent and was maximal at 10%. Also frozen GFs were able to stimulate proliferation. In conclusion we identified a method to promote ex-vivo expansion of MSC to be used in bone reconstruction.

Keywords

biochemistry; blood; bone; cellular biophysics; orthopaedics; surgery; bone marrow; bone reconstruction; clinical applications; clonogenic adherent marrow-derived cells subset; ex-vivo expanded cells; growth factors; human mesenchymal stem cells; multipotential cells; platelets; proliferation enhancement; Acceleration; Biological materials; Biological tissues; Bones; Humans; Laboratories; Muscles; Stem cells; Tendons; Testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Molecular, Cellular and Tissue Engineering, 2002. Proceedings of the IEEE-EMBS Special Topic Conference on

Print_ISBN

0-7803-7557-2

Type

conf

DOI

10.1109/MCTE.2002.1175052

Filename

1175052