Title :
PCR using electrostatic micromanipulation
Author :
Tokoro, Masaya ; Katayama, Tetsuo ; Taniguchi, Tomohiro ; Torii, Tom ; Higuchi, Toshiro
Author_Institution :
Dept. of Precision Eng., Univ. of Tokyo, Japan
Abstract :
Feasibility study of PCR using electrostatic manipulation of micro droplets is discussed. Adherence of droplets to the surface has been a difficult hindrance to overcome when controlling PCR solution in liquid medium. Different types of medium immiscible with the droplets are tested and n-octane enables electrostatic manipulation of PCR droplets. The concept of a novel micro PCR device is proposed.
Keywords :
biochemistry; drops; electrohydrodynamics; electrostatics; molecular biophysics; proteins; PCR solution control; droplet adherence; electrostatic micromanipulation; immiscible media; micro droplets; Chemical reactors; Electrodes; Electrostatics; Frequency; Lab-on-a-chip; Micropumps; Petroleum; Testing; Valves; Voltage;
Conference_Titel :
SICE 2002. Proceedings of the 41st SICE Annual Conference
Print_ISBN :
0-7803-7631-5
DOI :
10.1109/SICE.2002.1195296