A variable-focus lens with 1 kHz bandwidth applied to axial-scan of a confocal scanning microscope

Author

Oku, Hironiasa ; Ishikawa, Masatoshi

Author_Institution

Intelligent Cooperation & Control, Japan Sci. & Technol. Corp., Tokyo, Japan

Volume

1

fYear

2003

fDate

27-28 Oct. 2003

Firstpage

309

Abstract

This paper proposes a new structure of variable-focus lens aimed at 1 kHz bandwidth. Two kinds of prototypes are developed to validate the structure. The 1 kHz bandwidth is confirmed by the frequency response measurements. One prototype is applied to the axial high-speed scan of a confocal laser scanning microscope. Depth information measurements of a microscopic object in 1 ms and automated focusing to keep the focus on a randomly moving object are successfully achieved.

Keywords

biological techniques; high-speed optical techniques; laser beam applications; lenses; near-field scanning optical microscopy; optical focusing; optical variables measurement; 1 ms; automated focusing; axial-scan; confocal laser scanning microscope; confocal scanning microscope; depth information measurements; frequency response measurements; high-speed scan; microscopic object; randomly moving object; variable-focus lens; Actuators; Bandwidth; Focusing; Frequency measurement; Frequency response; Glass; Lenses; Optical design; Optical microscopy; Prototypes;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Society, 2003. LEOS 2003. The 16th Annual Meeting of the IEEE

ISSN

1092-8081

Print_ISBN

0-7803-7888-1

Type

conf

DOI

10.1109/LEOS.2003.1251762

Filename

1251762