Title :
Micro-spectroscopy of two-photon autofluorescence from PC12 cells
Author :
Perevedentseva, Elena ; Kao, Fu-Jen
Author_Institution :
Dept. of Phys., Nat. Sun Yat-Sen Univ., Kaohsiung, Taiwan
Abstract :
The two-photon autofluorescence from PC12 cells and cell medium components was investigated. Both the PC12 cells and the medium exhibit autofluorescence in the spectral regime of 505-530 nm. We have found that the autofluorescence intensity from cells depends sensitively on the cells´ conditions.
Keywords :
cellular biophysics; fluorescence spectroscopy; infrared spectra; two-photon processes; visible spectra; 503 to 530 nm; PC cell; autofluorescence intensity; cell medium component; micro-spectroscopy; spectral regime; two-photon autofluorescence; Filters; Fluorescence; Laser beam cutting; Laser excitation; Microscopy; Optical imaging; Pump lasers; Sea measurements; Solid lasers; Spectroscopy;
Conference_Titel :
Lasers and Electro-Optics, 2003. CLEO/Pacific Rim 2003. The 5th Pacific Rim Conference on
Print_ISBN :
0-7803-7766-4
DOI :
10.1109/CLEOPR.2003.1274764