Title :
Two-photon UV fluorescence excitation of the amino acids by a frequency doubled femtosecond optical parametric oscillator
Author :
Yang, Te-Chen ; Kao, Fu-Jen
Author_Institution :
Dept. of Phys., Nat. Sun Yat-Sen Univ., Kaohsiung, Taiwan
Abstract :
In this study, we have demonstrated the use of a frequency-doubled femtosecond optical parametric oscillator in generating two-photon excitation that is equivalent to ultraviolet (UV) light with wavelength less than 300 nm. This capability allows observation of some amino acids and enables excitation that is only possible with wavelength in UVB range (290 nm-320 nm).
Keywords :
fluorescence; high-speed optical techniques; optical harmonic generation; optical parametric oscillators; organic compounds; photoexcitation; two-photon processes; ultraviolet spectroscopy; 290 to 320 nm; amino acids; frequency doubled femtosecond optical parametric oscillator; two-photon UV fluorescence excitation; Amino acids; Biomedical optical imaging; Fluorescence; Frequency; Laser excitation; Nonlinear optics; Optical filters; Optical harmonic generation; Oscillators; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics, 2003. CLEO/Pacific Rim 2003. The 5th Pacific Rim Conference on
Print_ISBN :
0-7803-7766-4
DOI :
10.1109/CLEOPR.2003.1274816
