Title :
Laser scanning microscopy of enamel and dentin sections with two-photon fluorescence and second harmonic generation
Author :
Jian-Cheng Chen ; Fu-Jen Kao
Author_Institution :
Inst. of Electro-Opt. Eng., Nat. Sun Yat-Sen Univ., Kaohsiung, Taiwan
Abstract :
In this study, we have demonstrated the use of two-photon fluorescence and second harmonic (SH) generation in imaging the enamel and dentin sections. In addition, microspectroscopy is employed to identify the origin of two-photon fluorescent and SH signals, which are attributed to hydroxyapatite crystals and collagen fibrils, respectively. Hydroxyapatite is found to be present throughout the sections while collagen fibrils only exist in the dentin and dentinoenamel junctions.
Keywords :
biomedical optical imaging; dentistry; fluorescence; laser applications in medicine; molecular biophysics; optical harmonic generation; proteins; collagen fibrils; dentin; dentinoenamel junctions; enamel; hydroxyapatite crystals; laser scanning microscopy; second harmonic generation; two-photon fluorescence; Biomedical optical imaging; Crystals; Fluorescence; Frequency conversion; Nonlinear optics; Optical filters; Optical harmonic generation; Optical imaging; Optical microscopy; Power harmonic filters;
Conference_Titel :
Lasers and Electro-Optics, 2003. CLEO/Pacific Rim 2003. The 5th Pacific Rim Conference on
Print_ISBN :
0-7803-7766-4
DOI :
10.1109/CLEOPR.2003.1277229
