Laser scanning microscopy of enamel and dentin sections with two-photon fluorescence and second harmonic generation

Author

Jian-Cheng Chen ; Fu-Jen Kao

Author_Institution

Inst. of Electro-Opt. Eng., Nat. Sun Yat-Sen Univ., Kaohsiung, Taiwan

Volume

2

fYear

2003

fDate

15-19 Dec. 2003

Abstract

In this study, we have demonstrated the use of two-photon fluorescence and second harmonic (SH) generation in imaging the enamel and dentin sections. In addition, microspectroscopy is employed to identify the origin of two-photon fluorescent and SH signals, which are attributed to hydroxyapatite crystals and collagen fibrils, respectively. Hydroxyapatite is found to be present throughout the sections while collagen fibrils only exist in the dentin and dentinoenamel junctions.

Keywords

biomedical optical imaging; dentistry; fluorescence; laser applications in medicine; molecular biophysics; optical harmonic generation; proteins; collagen fibrils; dentin; dentinoenamel junctions; enamel; hydroxyapatite crystals; laser scanning microscopy; second harmonic generation; two-photon fluorescence; Biomedical optical imaging; Crystals; Fluorescence; Frequency conversion; Nonlinear optics; Optical filters; Optical harmonic generation; Optical imaging; Optical microscopy; Power harmonic filters;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2003. CLEO/Pacific Rim 2003. The 5th Pacific Rim Conference on

Print_ISBN

0-7803-7766-4

Type

conf

DOI

10.1109/CLEOPR.2003.1277229

Filename

1277229