Simultaneous measurement of ciliary beat frequency and wave disorder on respiratory epithelial cells

Author

Yi, W.J. ; Park, K.S. ; Lee, C.H. ; Rhee, C.S.

Author_Institution

Dept. of Biomed. Eng., Seoul Nat. Univ., South Korea

Volume

2

fYear

2003

fDate

17-21 Sept. 2003

Firstpage

1047

Abstract

Ciliary beat and its metachronism are fundamental to effective mucociliary transport. The beat frequencies (CBF) and metachronal wave directions of multiple cilia beating in culture media were measured simultaneously using digital microscopic images. The wave propagation directions of cilia were determined from a 2-dimensional correlation map by a principal axis method. The standard deviation of measured wave directions in a region of interest (ROI) was defined as a measure of metachronal wave disorder (MWD). Considerable variation was found in the beat frequencies and metachronal wave directions of cilia beating on epithelium. The pooled mean of MWDs was 23.4+/-8.79 ° and the pooled mean of CBFs 10.1+/-1.89Hz on 120 cells in mucosae of the sphenoid sinus from 5 healthy subjects.

Keywords

CCD image sensors; biomedical optical imaging; cellular biophysics; eye; optical microscopy; pneumodynamics; ciliary beat frequency; digital microscopic image; effective mucociliary transport; metachronal wave disorder; metachronism; mucosae; pooled mean; region of interest; respiratory epithelial cell; simultaneous measurement; sphenoid sinus; standard deviation; wave disorder; wave propagation direction; Biomedical engineering; Biomedical imaging; Biomedical measurements; Educational institutions; Frequency measurement; Heat engines; Heating; Measurement standards; Microscopy; Propulsion;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2003. Proceedings of the 25th Annual International Conference of the IEEE

ISSN

1094-687X

Print_ISBN

0-7803-7789-3

Type

conf

DOI

10.1109/IEMBS.2003.1279424

Filename

1279424