Title :
Intracellular oxygen sensing using a tunable UV-visible-NIR fluorescence lifetime imaging microscopy system
Author :
Zhong, Wei ; Urayama, Paul ; Mycek, Mary-Ann
Author_Institution :
Dept. of Phys. & Astron., Dartmouth Coll., Hanover, NH, USA
Abstract :
We present a fluorescence lifetime imaging microscopy system capable of excitation from UV to NIR wavelengths, developed for time-resolved biological fluorescence imaging. Intracellular ruthenium-based oxygen sensing is presented as an application.
Keywords :
biological techniques; cellular biophysics; fluorescence; infrared imaging; optical microscopy; optical tuning; oxygen; ruthenium; O; Ru; UV-visible-NIR wavelengths; fluorescence lifetime imaging microscopy system; intracellular ruthenium-based oxygen sensing; time-resolved biological fluorescence imaging; Extraterrestrial measurements; Fluorescence; Laser excitation; Mirrors; Nuclear measurements; Optical imaging; Optical microscopy; Optical sensors; Oxygen; Tunable circuits and devices;
Conference_Titel :
Lasers and Electro-Optics, 2003. CLEO '03. Conference on
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-748-2
