Synchronized manipulation and force measurement by optical tweezers using high-speed laser scanning

Laser micromanipulation is suitable for non-contact manipulation of a small object, such as a bacteria and microbe, at the closed space. We proposed synchronized laser micromanipulation (SLM) for the trajectory control of the multiple targets by the single laser. We used the laser scanning system for scanning of the focal point of laser in high speed to realize simultaneous trajectory control of multiple objects. Condition for secure transportation of multiple objects is derived analytically and verified through the experiment. We used SLM for indirect manipulation of the microbe by the microtools, which are directly manipulated by SLM. Here we propose the force measurement method using SLM. Recognition of the microtool is improved by using a fluorescent microbead as a microtool.