Optical imaging with retrogradely transported voltage-sensitive dye from projection neurons in marginal layer of the spinal dorsal horn

Projection neurons in the marginal layer of the spinal dorsal horn have been proposed to play an important role for central sensitization. Here, we report a novel method for imaging the neuronal activity from projection neurons to the brainstem in the marginal layer of the spinal dorsal horn by using a retrogradely transported voltage-sensitive dye.