Electrical detection of the contact between a microinjection pipette and cells

Author

Lukkari, M.J. ; Karjalainen, M.I. ; Sarkanen, R. ; Linne, M.-L. ; Jalonen, T.O. ; Kallio, P.J.

Author_Institution

Inst. of Autom. & Control, Tampere Univ. of Technol., Finland

Volume

1

fYear

2004

fDate

1-5 Sept. 2004

Firstpage

2557

Lastpage

2560

Abstract

This work presents the basic design and tests of a device designed for detecting the contact between a microinjection pipette and cell membrane. The device facilitates the automation of the microinjection procedure of living adherent cells. The measurement of the contact is based on measuring the resistance of the pipette. Breakage and clogging of the pipette can be detected with the same measurement.

Keywords

bioelectric phenomena; biomedical equipment; biomedical measurement; biomembranes; cellular biophysics; electrical contacts; cell membrane; electrical contact detection; living adherent cells; microinjection pipette; micromanipulator; pipette breakage; pipette clogging; resistance measurement; Clamps; Contacts; Current measurement; Electrical resistance measurement; Electrodes; Immune system; Microinjection; Micromanipulators; Pulse measurements; Voltage; Contact; microinjection; micromanipulator;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE

Conference_Location

San Francisco, CA

Print_ISBN

0-7803-8439-3

Type

conf

DOI

10.1109/IEMBS.2004.1403735

Filename

1403735