Title :
Hybrid polymer/thin film impedance system for label free monitoring of cells
Author :
Mohanty, Swomitra K. ; Sohn, Lydia L. ; Beebe, David J.
Author_Institution :
Dept. of Biomed. Eng., Wisconsin Univ., Madison, WI, USA
Abstract :
Impedance measurements (capacitive cytometry) have been used to perform label-free analysis of cells. Two devices were constructed using a simple liquid photopolymerization technique known as μFluidicTectonics. This platform has made it possible to rapidly fabricate with great ease and without the use of traditional MEMS technology a cell impedance measurement system. Measurements were made using not only a single 1 kHz frequency but also a frequency sweep from 50 Hz to 20 kHz. Single frequency measurements on SP2 mouse cells showed show that there is a strong, linear relationship between the DNA content of individual cells and their dielectric (or capacitance) response to a 1 kHz field. Frequency sweeps were conducted on SF9 cells as means to perform diagnostic measurements of the device when different number of cells are present.
Keywords :
DNA; biological techniques; capacitance measurement; cellular biophysics; electric impedance measurement; frequency measurement; polymerisation; /spl mu/FluidicTectonics; 1 kHz; 50 Hz to 20 kHz; DNA content; SP2 mouse cells; capacitance response; capacitive cytometry; dielectric response; hybrid polymer-thin film impedance system; impedance measurement; label free monitoring; liquid photopolymerization technique; single frequency measurement; DNA; Dielectric measurements; Frequency measurement; Impedance measurement; Mice; Micromechanical devices; Monitoring; Performance analysis; Performance evaluation; Polymer films; μFluidic Tectonics; capactive cytometry; impedance;
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Conference_Location :
San Francisco, CA
Print_ISBN :
0-7803-8439-3
DOI :
10.1109/IEMBS.2004.1403736
