An examination of the effect of an AC pulsed electric field on cell mortality in SWLA-2 hybridomas

This work describes the initial experimental setup and results involving the percentage cell lysis in SWLA-2 murine hybridomas produced by AC electric field pulses of varying amplitudes and pulse widths. Cells were cultured and separate samples examined at 24 hours. The frequency, pulse width and peak-to-peak voltage were varied. AC electric fields producing at least 1 V across the cell membrane appear to be more effective in producing cell lysis than similar fields producing lower membrane voltages. Additionally, higher frequencies, in the 10 kHz range, appear to be more effective than lower frequencies at membrane voltages above 1 V in producing cell lysis.