Title :
Imaging mitochondria in living cells by confocal fluorescence microscopy
Author :
Peng, Tsung-I ; Jou, Mei-Jie
Author_Institution :
Dept. of Neurology, Chung Gung Memorial Hosp., Tao-Yuan, Taiwan
Abstract :
Images of mitochondria inside living cells with high spatial and temporal resolution could be visualized with specific functional fluorescent probes coupled with laser scanning confocal microscope. The interactions between mitochondria and other parts of cells remain nature and unaltered with this method. The effects of mitochondrial function changes on other parts of cells can best be investigated with this approach.
Keywords :
biomedical optical imaging; cellular biophysics; fluorescence; image resolution; optical microscopy; scanning probe microscopy; confocal fluorescence microscopy; functional fluorescent probes; image resolution; laser scanning confocal microscope; living cells; mitochondria imaging; Biochemistry; Calcium; Condition monitoring; Fluorescence; Hospitals; Microscopy; Nervous system; Pathology; Probes; Spatial resolution;
Conference_Titel :
Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on
Print_ISBN :
0-7803-8676-0
DOI :
10.1109/APBP.2004.1412293
