An investigation of the relationship between the transdermal drug delivery and membranes fluidity by multiphoton imaging of Laurdan

Multiphoton microscopy is a three-dimensional imaging technique with a number of advantages. For example, multiphoton microscopy can image tissues up to a depth of a few hundred micrometers, at high resolution and with improved contrast. In this work, we used multiphoton microscopy in investigating the actions of the chemical enhancer oleic acid in transdermal drug delivery. Technique of generalized polarization (GP) imaging and polarization (P) imaging associate with Laurdan to monitor the lipid layer changes caused by the addition of oleic acid. Our study will help to develop more effective methodologies in the delivering of molecules across biological barriers.