DocumentCode :
430467
Title :
Evanescent planar wave system for reading DNA microarrays on thin glass slides
Author :
Wei, Pei-Kuen ; Huang, Peng-Cheng ; Chen, Yi-Chun
Author_Institution :
Res. Center for Appl. Sci., Acad. Sinica, Taipei, Taiwan
fYear :
2004
fDate :
14-17 Dec. 2004
Firstpage :
236
Lastpage :
237
Abstract :
A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.
Keywords :
DNA; biomedical optical imaging; diffraction gratings; fluorescence; laser beam applications; molecular biophysics; polymer films; DNA microarray; PDMS film; TIR; detection area; evanescent planar wave system; excitation background; fluorescent signal; grating coupling; normally incident laser; optical reading system; thin glass slide; total internal reflection; DNA; Fluorescence; Glass; Gratings; Optical coupling; Optical films; Optical reflection; Optical sensors; Optical surface waves; Surface waves;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on
Print_ISBN :
0-7803-8676-0
Type :
conf
DOI :
10.1109/APBP.2004.1412369
Filename :
1412369
Link To Document :
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