Agarose Gel Investigation of Quantum Dots Conjugated With Short ssDNA

Author

Tsai-Chin Wu ; Dutta, Maitreyee ; Stroscio, Michael A.

Author_Institution

Dept. of Bioeng., Univ. of Illinois at Chicago, Chicago, IL, USA

Volume

12

Issue

4

fYear

2013

fDate

Dec. 2013

Firstpage

282

Lastpage

288

Abstract

Herein, we investigate the migration distance of quantum-dot-functionalized complexes in electrophoresis. The quantitative study of these moving particles in an electrophoretic environment is modeled using an extended Smoluchowski equation. An extended Smoluchowski equation is proposed to addressed the D_m to Ln(N) plot slope variation issue present in previous work and agreement between experiment and theory is found. The procedures underlying this work then discusses the potential of using agarose electrophoresis as a mean of monitoring the composition of nano-complexes consisting of quantum dots functionalized with differing numbers of DNA molecules.

Keywords

DNA; biochemistry; biological techniques; electrophoresis; molecular biophysics; nanostructured materials; quantum dots; DNA molecules; agarose electrophoresis; agarose gel investigation; electrophoretic environment; extended Smoluchowski equation; migration distance; moving particles; nanocomplex composition; nanocomplex monitoring; plot slope variation; quantum dot; quantum-dot-functionalized complexes; short ssDNA; DNA; Molecular biophysics; Quantum dots; DNA; Smoluchowski equation; double layer; gel electrophoresis; quantum dots;

fLanguage

English

Journal_Title

NanoBioscience, IEEE Transactions on

Publisher

ieee

ISSN

1536-1241

Type

jour

DOI

10.1109/TNB.2013.2268540

Filename

6698363