Glucose Oxidase (GOD)-Coupled Amperometric Microsensor with Integrated Electrochemical Actuation System

Recent developments for biosensors have been mainly focused on miniaturization and exploratory use of new materials. It should be emphasized that the absence of a novel "in-situ self-calibration/diagnosis technique" that is not connected to an external apparatus is a key obstacle to the realization of a biosensor for continuous use with minimum attendance. In order to address this issue, a novel solid-state glucose oxidase-coupled amperometric biosensor with integrated electrochemical actuation system has been designed and validated. There are two key components of the proposed glucose biosensor: solid-state GOD-coupled thin-1m amperometric sensing element and O₂ depleting/saturating built-in electrochemical actuator. The actuator can be used to accomplish in-situ 1-point self-calibration by depleting O₂ (i.e., by simulating glucose-free environment). Also, it can be used at the same time to extend the proposed sensor\´s linear detection range by increasing O₂ (i.e., by enhancing glucose sensitivity). A prototype sensor was fabricated and a series of lab experiments was conducted. Collected data assures that the proposed sensor effectively establishes the zero calibration point and significantly enhances its measurement sensitivity and confidence