Title :
Electronic Gate Detection for the Counting and Sizing of Cells or Colloidal Particles in Liquids: Front-end Characteristics, Specific Problems and their Remediation. Part 1: Physical Model and Equivalent Circuits.
Author :
De Bisschop, F.R.
Author_Institution :
Fac. of Medicine, Ghent Univ., Gent
Abstract :
DC, RF and LF operated gating for cell/particle counting and sizing in liquids are hindered by unstable gate impedance. Most problematic appear transients and fluctuations due to varying flow patterns of the carrier liquid. Their effects, largely hidden in DC-operated gating, cause broadening and shift of cell/particle size distributions. RF/LF-operated gating, more specifically the demodulation of the AM-encoded gate signal, is much more disturbed. For a systematic study of these effects, an equivalent circuit is presented along with a procedure to determine the system parameters
Keywords :
biomedical measurement; cellular biophysics; equivalent circuits; flow measurement; particle size; AM-encoded gate signal; DC-operated gating; RF/LF-operated gating; cell counting; cell sizing; cell/particle size distributions; colloidal particles; electronic gate detection; equivalent circuits; front-end characteristics; particle counting; particle sizing; unstable gate impedance; Band pass filters; Demodulation; Electrodes; Equivalent circuits; Impedance; Liquids; Low-frequency noise; Pulse measurements; RF signals; Radio frequency; Electronic Gating; Flow Dependent Gate Impedance; Flow cytometry;
Conference_Titel :
Instrumentation and Measurement Technology Conference, 2005. IMTC 2005. Proceedings of the IEEE
Conference_Location :
Ottawa, Ont.
Print_ISBN :
0-7803-8879-8
DOI :
10.1109/IMTC.2005.1604383
