Serum albumin measurement using a tapered fluorescent fiber-optic evanescent wave-based biosensor

A novel method to measure the total serum protein concentration is described in this paper. The method uses a tapered fiber optics based on the principles of fiber optic evanescent wave spectroscopy. The biosensor applies a commercially available fluorescent dye-immobilized porous glass coating on a multi-mode optical fiber. The evanescent waves at the fiber optic core-cladding interface are used to monitor the protein-induced changes in the sensor element. The sensor offers a rapid, single-step method for quantifying protein concentrations without destroying the sample. This unique sensing method presents a sensitive and accurate platform for the quantification of protein.